Anti-PARK7/DJ1 antibody
| Name | Anti-PARK7/DJ1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab18257 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Rabbit, Human, Bovine, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human PARK7/DJ1 |
| Blocking Peptide | Human PARK7/DJ1 peptide |
| Description | Rabbit Polyclonal |
| Gene | PARK7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-PARK7/DJ1 antibody (ab18257) at 1 µg/mlLane 1 : Jurkat lysateLane 2 : HeLa lysateLane 3 : 3T3 lysateLane 4 : Jurkat lysate with Human PARK7/DJ1 peptide (ab18659) at 1 µg/mlLane 5 : HeLa lysate with Human PARK7/DJ1 peptide (ab18659) at 1 µg/mlLane 6 : 3T3 lysate with Human PARK7/DJ1 peptide (ab18659) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG (680) at 1/10000 dilution
ICC/IF image of ab18257 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18257, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
All lanes : Anti-PARK7/DJ1 antibody (ab18257) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Liver (Mouse) Tissue Lysate - normal tissueLane 3 : Heart (Mouse) Tissue Lysate Lane 4 : Kidney (Mouse) Tissue LysateLane 5 : Pancreas (Mouse) Tissue Lysate (ab29363)Lane 6 : Testis (Mouse) Tissue Lysate - normal tissue Lane 7 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)Lane 8 : Spinal Cord (Mouse) Tissue LysateLane 9 : Ovary (Mouse) Tissue Lysate - normal tissue Lane 10 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 11 : Brain (Rat) Tissue Lysate - normal tissue Lane 12 : Liver (Rat) Tissue Lysate Lane 13 : Heart (Rat) Tissue Lysate Lane 14 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
Image courtesy of Human Protein Atlasab18257 staining PARK7/DJ1 in Human parathyroid. The paraffin embedded tissue was incubated with ab18257 (1/22000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab18257 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.Further results for this antibody can be found at www.proteinatlas.org
Anti-PARK7/DJ1 antibody (ab18257) at 1 µg/ml + Human PARK7/DJ1 full length protein (ab51198) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute
Product References
Salivary DJ-1 could be an indicator of Parkinson's disease progression. - Salivary DJ-1 could be an indicator of Parkinson's disease progression.
Kang WY, Yang Q, Jiang XF, Chen W, Zhang LY, Wang XY, Zhang LN, Quinn TJ, Liu J, Chen SD. Front Aging Neurosci. 2014 Jun 6;6:102.
DJ-1 interactions with alpha-synuclein attenuate aggregation and cellular - DJ-1 interactions with alpha-synuclein attenuate aggregation and cellular
Zondler L, Miller-Fleming L, Repici M, Goncalves S, Tenreiro S, Rosado-Ramos R, Betzer C, Straatman KR, Jensen PH, Giorgini F, Outeiro TF. Cell Death Dis. 2014 Jul 24;5:e1350.
DJ-1 interacts with and regulates paraoxonase-2, an enzyme critical for neuronal - DJ-1 interacts with and regulates paraoxonase-2, an enzyme critical for neuronal
Parsanejad M, Bourquard N, Qu D, Zhang Y, Huang E, Rousseaux MW, Aleyasin H, Irrcher I, Callaghan S, Vaillant DC, Kim RH, Slack RS, Mak TW, Reddy ST, Figeys D, Park DS. PLoS One. 2014 Sep 11;9(9):e106601.
ER-stress-associated functional link between Parkin and DJ-1 via a - ER-stress-associated functional link between Parkin and DJ-1 via a
Duplan E, Giaime E, Viotti J, Sevalle J, Corti O, Brice A, Ariga H, Qi L, Checler F, Alves da Costa C. J Cell Sci. 2013 May 1;126(Pt 9):2124-33.
RNA silencing of genes involved in Alzheimer's disease enhances mitochondrial - RNA silencing of genes involved in Alzheimer's disease enhances mitochondrial
Manczak M, Reddy PH. Biochim Biophys Acta. 2013 Dec;1832(12):2368-78. doi:
YajL, prokaryotic homolog of parkinsonism-associated protein DJ-1, functions as a - YajL, prokaryotic homolog of parkinsonism-associated protein DJ-1, functions as a
Le HT, Gautier V, Kthiri F, Malki A, Messaoudi N, Mihoub M, Landoulsi A, An YJ, Cha SS, Richarme G. J Biol Chem. 2012 Feb 17;287(8):5861-70.
DJ-1 promotes invasion and metastasis of pancreatic cancer cells by activating - DJ-1 promotes invasion and metastasis of pancreatic cancer cells by activating
He X, Zheng Z, Li J, Ben Q, Liu J, Zhang J, Ji J, Yu B, Chen X, Su L, Zhou L, Liu B, Yuan Y. Carcinogenesis. 2012 Mar;33(3):555-62.
MicroRNA profiling of Parkinson's disease brains identifies early downregulation - MicroRNA profiling of Parkinson's disease brains identifies early downregulation
Minones-Moyano E, Porta S, Escaramis G, Rabionet R, Iraola S, Kagerbauer B, Espinosa-Parrilla Y, Ferrer I, Estivill X, Marti E. Hum Mol Genet. 2011 Aug 1;20(15):3067-78.
DJ-1 can inhibit microtubule associated protein 1 B formed aggregates. - DJ-1 can inhibit microtubule associated protein 1 B formed aggregates.
Wang Z, Zhang Y, Zhang S, Guo Q, Tan Y, Wang X, Xiong R, Ding J, Chen S. Mol Neurodegener. 2011 Jun 6;6:38.
Loss of function of DJ-1 triggered by Parkinson's disease-associated mutation is - Loss of function of DJ-1 triggered by Parkinson's disease-associated mutation is
Giaime E, Sunyach C, Druon C, Scarzello S, Robert G, Grosso S, Auberger P, Goldberg MS, Shen J, Heutink P, Pouyssegur J, Pages G, Checler F, Alves da Costa C. Cell Death Differ. 2010 Jan;17(1):158-69.