All lanes : Anti-Parkin antibody [EPR5024(N)] (ab179812) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Parkin with ab179812 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab179812 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized Jurkat cells labeling Parkin with ab179812 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Parkin with ab179812 at 1/50 dilution.