Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PARP10 with ab70800 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-PARP10 antibody (ab70800) at 1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique
1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70800 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of immunoprecipitate was loaded per lane, and probed with ab70800 at 1ug/ml. Detection: chemiluminescence with exposure time of 30 seconds.