Anti-Pax2 antibody
| Name | Anti-Pax2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38738 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Zebrafish, Chicken, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Pax2 |
| Description | Rabbit Polyclonal |
| Gene | PAX2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC-Fr image of Pax2 staining on zebrafish retina sections using ab38738 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab38738 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).See Abreview
All lanes : Anti-Pax2 antibody (ab38738) at 1/250 dilutionLane 1 : Mouse Fetal lysate - 14 Days old Tissue Lysate Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)Lane 3 : Mouse Embryonic Fibroblast 1 Whole Cell LysateLane 4 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab38738 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38738, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Gene expression profiling reveals differentially expressed genes in ovarian - Gene expression profiling reveals differentially expressed genes in ovarian
Trevino LS, Giles JR, Wang W, Urick ME, Johnson PA. Horm Cancer. 2010 Aug;1(4):177-86.
Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. - Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen.
Hurtado A, Holmes KA, Geistlinger TR, Hutcheson IR, Nicholson RI, Brown M, Jiang J, Howat WJ, Ali S, Carroll JS. Nature. 2008 Dec 4;456(7222):663-6.