Anti-PAX8 antibody [1F8-3A8]

• Supplier: Abcam
• Catalog: ab183573
• Prices: $376.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 1F8-3A8
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Human, Primate
• Antigen: Recombinant fragment corresponding to Human PAX8 (internal sequence)
• Description: Mouse Monoclonal
• Gene: PAX8
• Conjugate: Unconjugated

Product images

All lanes : Anti-PAX8 antibody [1F8-3A8] (ab183573) at 1/1000 dilutionLane 1 : SKOV-3 cell lysateLane 2 : COS7 cell lysateLane 3 : Negative control HeLa whole cell lysatesLysates/proteins at 60 µg per lane.SecondaryHRP-conjugated goat anti-mouse IgG at 1/500 dilution

Immunofluorescent analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel) using ab183573 at a 1/50 dilution. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% blocker BSA for 15 minutes at room temperature.  A DyLight 488-conjugated goat anti-mouse IgG secondary antibody was used at a dilution of 1/400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

All lanes : Anti-PAX8 antibody [1F8-3A8] (ab183573) at 1/1000 dilutionLane 1 : untransfected HEK293T cell lysateLane 2 : HEK293T cell lysate over expressing PAX8Lysates/proteins at 25 µg per lane.SecondaryHRP-conjugated goat anti-mouse IgG at 1/500 dilution