Anti-PAX8 antibody [PAX8R1]

• Supplier: Abcam
• Catalog: ab53490
• Prices: $392.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: PAX8R1
• Applications: FC IHC-P WB IP ICC/IF IHC-F
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant C-terminal fragment corresponding to Human PAX8
• Description: Mouse Monoclonal
• Gene: PAX8
• Conjugate: Unconjugated

Product images

ab53490 staining PAX8 in Mouse thyroid tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/20 in 10% goat serum in PBS) for 1 hour at 24°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + HeLa whole cell lysate at 50 µgSecondaryMouse IgG antibody at 1/2500 dilution

Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µgSecondaryMouse IgG antibody at 1/2500 dilution

Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + F2408 whole cell lysate at 25 µgSecondaryMouse IgG antibody at 1/2500 dilution

ab53490, at a 1/10 dilution, staining PAX8 in HeLa cells, using Immunocytochemistry, plus AlexaFluor®488 Goat Anti-mouse IgG secondary anitbody.

Immunoprecipitation: ab53490: 5µg, RIPA lysate of HeLa cells: 500µg. WB: ab53490 at a 1:100 dilution, Secondary antibody - anti-mouse IgG at a 1:1000 dilution

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with ab53490 at 0.5 µg/sample (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG (0.25µg)

IHC image of ab53490 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Primary mucinous cystadenoma of the spermatic cord within the inguinal canal. - Primary mucinous cystadenoma of the spermatic cord within the inguinal canal.

Kim JY, Lee YT, Kang HJ, Lee CH. Diagn Pathol. 2012 Oct 8;7:139.

Immunohistochemical assessment of Pax8 expression during pancreatic islet - Immunohistochemical assessment of Pax8 expression during pancreatic islet

Lorenzo PI, Jimenez Moreno CM, Delgado I, Cobo-Vuilleumier N, Meier R, Gomez-Izquierdo L, Berney T, Garcia-Carbonero R, Rojas A, Gauthier BR. Histochem Cell Biol. 2011 Nov;136(5):595-607.

Paired box gene 8, HBME-1, and cytokeratin 19 expression in preoperative - Paired box gene 8, HBME-1, and cytokeratin 19 expression in preoperative

Schmitt AC, Cohen C, Siddiqui MT. Cancer Cytopathol. 2010 Aug 25;118(4):196-202.