Anti-PBK (phospho T9) antibody [EPR2475(N)]

• Supplier: Abcam
• Catalog: ab184953
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR2475(N)
• Applications: IHC-P FC WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PBK aa 1-100 (phospho T9)
• Description: Rabbit Monoclonal
• Gene: PBK
• Conjugate: Unconjugated

Product images

All lanes : Anti-PBK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilutionLane 1 : Hela lysate treated with NocodazoleLane 2 : Hela lysate untreatedLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PBK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling PBK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin

Flow cytometric analysis of 2% paraformaldehyde-fixed, permeabilized HeLa cells treated with Nocodazole labeling PBK (phospho T9) with ab184953 at 1/100 dilution (red) compared to untreated HeLa cells (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. Nocodazole-treated HeLa cells labeled using ab184953 preincubated with phospho PBK (phospho T9) peptide (blue) or non phosphopeptide (orange).