Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreatic islet cell tumor tissue labeling PBXIP1 with ab176591 at 1µg/ml.
All lanes : Anti-PBXIP1 antibody - N-terminal (ab176591) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique
Detection of PBXIP1 by Western Blot of Immunprecipitate.ab176591 at 0.1µg/ml labeling PBXIP1 in HeLa whole cell lysate immunoprecipitated using ab176591 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.