Anti-PCBP1 antibody
| Name | Anti-PCBP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab74793 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Mouse, Rat, Human, Rabbit, Horse, Guinea Pig, Bovine, Cat, Dog |
| Antigen | A synthetic peptide corresponding to a region of Human PCBP1 |
| Description | Rabbit Polyclonal |
| Gene | PCBP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab74793 staining PCBP1 in Human breast Tissue by Immunohistochemistry (Paraffin-embedded sections). Primary antibody used at 5 μg/ml
ab74793 staining PCBP1 in Human spleen Tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) after heat treatment antigen retrieval. Primary antibody used at 5 μg/ml
Anti-PCBP1 antibody (ab74793) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab74793 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74793, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab74793 at 5µg/ml staining PCBP1 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue).
All lanes : Anti-PCBP1 antibody (ab74793) at 1 µg/mlLane 1 : Whole cell lysate prepared from MEF cellsLane 2 : Whole cell lysate prepared from F9 cellsLane 3 : Whole cell lysate prepared from GC1 cellsLane 4 : Whole cell lysate prepared from NIH-3T3 cellsLysates/proteins at 30 µg per lane.SecondaryHRP conjugated pig anti-rabbit polyclonaldeveloped using the ECL technique
Product References
Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1 - Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1
Ho JJ, Robb GB, Tai SC, Turgeon PJ, Mawji IA, Man HS, Marsden PA. Mol Cell Biol. 2013 May;33(10):2029-46.
Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial - Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial
Sutherland JM, Keightley RA, Nixon B, Roman SD, Robker RL, Russell DL, McLaughlin EA. J Cell Physiol. 2012 Mar;227(3):1188-98.
Proteomic profile of primary isolated rat mesangial cells in high-glucose culture - Proteomic profile of primary isolated rat mesangial cells in high-glucose culture
Li Z, Zhang H, Dong X, Burczynski FJ, Choy P, Yang F, Liu H, Li P, Gong Y. Biochem Cell Biol. 2010 Aug;88(4):635-48.