Anti-PCCB antibody
| Name | Anti-PCCB antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70416 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Human |
| Antigen | Full length human PCCB protein (NP_000523 |
| Description | Mouse Polyclonal |
| Gene | PCCB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-PCCB antibody (ab70416) at 1/500 dilution + human liver tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
All lanes : Anti-PCCB antibody (ab70416) at 1/500 dilutionLane 1 : PCCB transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
IHC image of ab70416 staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.