Confocal immunofluorescent analysis of U-251MG cells using ab170812 at a 1/10 dilution, followed by a goat anti-rabbit lgG conjugated to dye (green). DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-PCDH1 antibody - N-terminal (ab170812) at 1/100 dilutionLane 1 : MDA-MB453 cell line lysatesLane 2 : ZR-75-1 cell line lysatesLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling PCDH1 with ab170812 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.