All lanes : Anti-PCNA antibody - Nuclear Loading Control (ab139696) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µgLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µgLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µgLane 4 : Human PCNA full length protein (ab85651) at 0.2 µgSecondaryGoat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.