All lanes : Anti-PCTP antibody [EPR15390] (ab184944) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : K562 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Flow cytometry analysis of paraformaldehyde-fixed K562 cells labeling PCTP with ab184944 at a 1/200 dilution (Red), with a negative control rabbit IgG (Green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution.
Western blot analysis on immunoprecipitation from Human fetal liver lysate (Lane 1), labeling PCTP using ab184944 at 1/50 dilution Lane 2 shows PBS negative control. Secondary antibody, HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.