All lanes : Anti-PDE3A antibody, prediluted (ab125676) at 1/2000 dilutionLane 1 : COS cell lysate, non-transfectedLane 2 : COS cell lysate, transfected with Human PDE1A3 Lane 3 : COS cell lysate, transfected with Human PDE2A3 Lane 4 : COS cell lysate, transfected with Human PDE3A Lane 5 : COS cell lysate, transfected with Human PDE3B Lane 6 : COS cell lysate, transfected with Human PDE4D2 Lane 7 : COS cell lysate, transfected with Human PDE5A1 Lane 8 : COS cell lysate, transfected with Human PDE5A3 Lane 9 : COS cell lysate, transfected with Human PDE7A1 Lane 10 : COS cell lysate, transfected with Human PDE8A1 Lane 11 : COS cell lysate, transfected with Human PDE10A1 Lane 12 : COS cell lysate, transfected with Human PDE11A1