Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling PDI with ab180931 at 1/100 dilution (green) or Rabbit monoclonal IgG (red), followed with Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
All lanes : Anti-PDI antibody [EPR12212] (ab180931) at 1/20000 dilutionLane 1 : Human pancreas lysateLane 2 : HeLa cell lysateLane 3 : BxPC-3 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution