All lanes : Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982) at 1 µg/mlLane 1 : Molecular weight markerLane 2 : Human PDP2 full length protein (ab110358) at 0.01 µgLane 3 : Human PDP1 full length protein (ab110357) at 0.01 µgLane 4 : Active human Mitochondrial Pyruvate dehydrogenase kinase 1 full length protein (ab110359) at 0.01 µgLane 5 : HHH (Human heart homogenate) at 20 µgLane 6 : HLH (Human liver homogenate) at 20 µgLane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgLane 8 : HEK293 (Human embryonic kidney 293 cell line) Whole Cell Lysate at 10 µgLane 9 : HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 10 µgLane 10 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.