All lanes : Anti-PDPK1 (phospho S241) antibody (ab131098) at 1/500 dilutionLane 1 : 293 cell extract, untreatedLane 2 : 293 cell extract treated with EGFLane 3 : PC12 cell extract, untreatedLane 4 : PC12 cell extract treated with EGF
Immunohistochemical analysis of PDPK1 (phospho S241) in paraffin embedded Human breast carcinoma tissue, using ab131098 at a dilution of 1/50. Image on the right was preincubated with blocking peptide.
Immunofluorescence analysis of PDPK1 (phospho S241) in methanol fixed HeLa cells, using ab131098 at a dilution of 1/100.