Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and prostate carcinoma (right) tissues labelling PDS5B with ab70298 at 1/200 (1µg/ml) and 1/80 (2.5µg/ml). Detection: DAB and DyLight® 594-conjugated goat anti-rabbit IgG (1/100).
All lanes : Anti-PDS5B antibody (ab70298) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µg
Western Blot showing ab70298 at 0.1µg/ml staining PDS5B in HeLa whole cell lysates immunoprecipitated using ab70298 at 3µg/mg lysate (1 mg/IP loaded/lane).