Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryngeal squamous cell carcinoma tissue labelling PDS5B with ab70299 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-PDS5B antibody (ab70299) at 0.04 µg/mlLane 1 : Jurkat cell lysate at 50 µgLane 2 : Jurkat cell lysate at 15 µgLane 3 : Jurkat cell lysate at 5 µgLane 4 : 293T cell lysate at 50 µgLane 5 : NIH3T3 cell lysate at 50 µgLane 6 : 293T cell lysate at 50 µgLane 7 : NIH3T3 cell lysate at 50 µg
Western Blot showing ab70299 at 1µg/ml staining PDS5B in HeLa whole cell lysates immunoprecipitated using Lane 1: ab70298 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 2: ab70299 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 3: control IgG was used for immunoprecipitation. Detection: Chemiluminescence with exposure time of 10 seconds.