Immunohistochemical analysis of formalin-fixed, paraffin embedded Human kidney (tubules) tissue labelling PEA15 with ab133217 at 10 ug/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
All lanes : Anti-PEA15 antibody (ab133217) at 1/500 dilutionLane 1 : Jurkat cell extract, treated with PMA 125 ng/ml 30'Lane 2 : Jurkat cell extract, treated with PMA 125 ng/ml 30' with synthesized peptide
Immunofluorescent analysis of HeLa cells labelling PEA15 with ab133217 at 1/100 dilution. The image on the right is treated with the synthesized peptide.