All lanes : Anti-Pea3 antibody (ab98068) at 1 µg/mlLane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 8 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.