Anti-Pea3 antibody - C-terminal (ab135590) at 1/50 dilution + Mouse heart tissue lysates at 35 µg
All lanes : Anti-Pea3 antibody - C-terminal (ab135590) at 1/50 dilutionLane 1 : 293 cell lysates, non-transfected Lane 2 : 293 cell lysates transiently transfected with Pea3 Lysates/proteins at 2 µg per lane.
Immunohistochemical analysis of formalin fixed and paraffin embedded Human brain tissue labelling Pea3 with ab135590 at a a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of widr cells (bottom histogram) labelling Pea3 with ab135590 at a 1/10 dilution, compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.