ab47638 staining Perilipin 3 in Human HUVEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
Lane 1 : Anti-Perilipin 3 antibody (ab47638) at 0.5 µg/mlLane 2 : Anti-Perilipin 3 antibody (ab47638) at 1 µg/mlLane 1 : Daudi cell lysateLane 2 : Daudi cell lysateLysates/proteins at 15 µg per lane.
ab47638 at 10 µg/ml staining M6RBP1 in rat liver tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Immunofluorescence of TIP47 in Rat Liver cells using ab47638 at 20 ug/ml.