Anti-Peripherin antibody - nociceptive neuron marker

• Supplier: Abcam
• Catalog: ab4666
• Prices: $400.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-F ICC/IF IHC-P IHC
• Species Reactivities: Mouse, Rat, Rabbit, Cat, Human
• Antigen: Recombinant full length rat peripherin purified from E
• Description: Rabbit Polyclonal
• Gene: PRPH
• Conjugate: Unconjugated

Product images

Anti-Peripherin antibody - nociceptive neuron marker (ab4666) at 1/20000 dilution + Whole rat brain stem homogenateObserved band size : 57 kDa (why is the actual band size different from the predicted?)Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

ab4666 at a 1/3000 dilution staining Peripherin in human small intestine tissue sections by Immunohistochemistry (paraffin embedded) incubated for 20 minutes at 25°C. Fixed in formaldehyde. Heat mediated antigen retrieval step performed using EDTA pH 8.0, for 20 minutes at 100°C. Secondary used undiluted polyclonal Goat anti-mouse/rabbit IgG.Note nerve fibers and ganglion cells in the submucosa, and between the circular and longitudinal muscle layersSee Abreview

ab4666 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab4666 used at 1/200 dilution overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Salt-inducible kinase 3, SIK3, is a new gene associated with hearing. - Salt-inducible kinase 3, SIK3, is a new gene associated with hearing.

Wolber LE, Girotto G, Buniello A, Vuckovic D, Pirastu N, Lorente-Canovas B, Rudan I, Hayward C, Polasek O, Ciullo M, Mangino M, Steves C, Concas MP, Cocca M, Spector TD, Gasparini P, Steel KP, Williams FM. Hum Mol Genet. 2014 Dec 1;23(23):6407-18.

The effects of bilateral common carotid artery occlusion on expression of - The effects of bilateral common carotid artery occlusion on expression of

Zhao X, Zhang H, Guo J, Zhang Y. Brain Res. 2013 Jan 23;1491:167-75.

Characterization of neuronal populations in the human trigeminal ganglion and - Characterization of neuronal populations in the human trigeminal ganglion and

Flowerdew SE, Wick D, Himmelein S, Horn AK, Sinicina I, Strupp M, Brandt T, Theil D, Hufner K. PLoS One. 2013 Dec 19;8(12):e83603.

Sortilin associates with Trk receptors to enhance anterograde transport and - Sortilin associates with Trk receptors to enhance anterograde transport and

Vaegter CB, Jansen P, Fjorback AW, Glerup S, Skeldal S, Kjolby M, Richner M, Erdmann B, Nyengaard JR, Tessarollo L, Lewin GR, Willnow TE, Chao MV, Nykjaer A. Nat Neurosci. 2011 Jan;14(1):54-61.

Adipose-derived stem cells stimulate regeneration of peripheral nerves: BDNF - Adipose-derived stem cells stimulate regeneration of peripheral nerves: BDNF

Lopatina T, Kalinina N, Karagyaur M, Stambolsky D, Rubina K, Revischin A, Pavlova G, Parfyonova Y, Tkachuk V. PLoS One. 2011 Mar 14;6(3):e17899.

Human CD271-positive melanoma stem cells associated with metastasis establish - Human CD271-positive melanoma stem cells associated with metastasis establish

Civenni G, Walter A, Kobert N, Mihic-Probst D, Zipser M, Belloni B, Seifert B, Moch H, Dummer R, van den Broek M, Sommer L. Cancer Res. 2011 Apr 15;71(8):3098-109.

Nav1.7 is the predominant sodium channel in rodent olfactory sensory neurons. - Nav1.7 is the predominant sodium channel in rodent olfactory sensory neurons.

Ahn HS, Black JA, Zhao P, Tyrrell L, Waxman SG, Dib-Hajj SD. Mol Pain. 2011 May 10;7:32.

Breathing without CO(2) chemosensitivity in conditional Phox2b mutants. - Breathing without CO(2) chemosensitivity in conditional Phox2b mutants.

Ramanantsoa N, Hirsch MR, Thoby-Brisson M, Dubreuil V, Bouvier J, Ruffault PL, Matrot B, Fortin G, Brunet JF, Gallego J, Goridis C. J Neurosci. 2011 Sep 7;31(36):12880-8.