Anti-Peroxiredoxin 1 antibody [EPR5434]

• Supplier: Abcam
• Catalog: ab109506
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5434
• Applications: WB IP ICC/IF ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: ASynthetic peptide corresponding to residues near the C-terminus of Human Peroxiredoxin 1
• Description: Rabbit Monoclonal
• Gene: PRDX1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Peroxiredoxin 1 antibody [EPR5434] (ab109506) at 1/10000 dilutionLane 1 : 293T cell lysate Lane 2 : K562 cell lysate Lane 3 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of Peroxiredoxin 1 expression in formalin-fixed, paraffin-embedded Human liver tissue using ab109506 at 1/100 dilution.

Immunohistochemical analysis of Peroxiredoxin 1 in formalin-fixed, paraffin-embedded Human kidney tissue using ab109506 at 1/100 dilution.

ICC/IF image of ab109506 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109506, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.