Anti-Peroxiredoxin 5 antibody [3F11]

• Supplier: Abcam
• Catalog: ab16944
• Prices: $394.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 3F11
• Applications: ICC/IF ICC/IF ELISA WB IP
• Species Reactivities: Mouse, Human
• Antigen: Recombinant fragment (Human)
• Description: Mouse Monoclonal
• Gene: PRDX5
• Conjugate: Unconjugated

Product images

ab16944 (1/1000) detection of Peroxiredoxin 5 in...Lane 1: HeLa cell lysateLane 2: 293T cell lysateLane 3: Jurkat cell lysate

ICC/IF image of ab16944 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16944, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Circadian clocks in human red blood cells. - Circadian clocks in human red blood cells.

O'Neill JS, Reddy AB. Nature. 2011 Jan 27;469(7331):498-503.

Role of superoxide-nitric oxide interactions in the accelerated age-related loss - Role of superoxide-nitric oxide interactions in the accelerated age-related loss

Sakellariou GK, Pye D, Vasilaki A, Zibrik L, Palomero J, Kabayo T, McArdle F, Van Remmen H, Richardson A, Tidball JG, McArdle A, Jackson MJ. Aging Cell. 2011 Oct;10(5):749-60.

Characterization of the placental macrophage secretome: implications for - Characterization of the placental macrophage secretome: implications for

Garcia K, Garcia V, Perez Laspiur J, Duan F, Melendez LM. Placenta. 2009 Feb;30(2):149-55.