Immunoprecipitation analysis of Peroxiredoxin 5 pulled down from HeLa cell lysates using ab180587 at 1/50 dilution.
Flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells labeling Peroxiredoxin 5 with ab180587 at 1/100 (pink). Control shown in green and secondary antibody was Goat anti rabbit IgG (FITC) at 1/150.
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Peroxiredoxin 5 with ab180587 at 1/250.
All lanes : Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilutionLane 1 : HepG2 cell line lysateLane 2 : Jurkat cell line lysateLane 3 : A549 cell line lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/20000 dilution + HeLa cell line lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution