Anti-Peroxiredoxin 6 antibody

• Supplier: Abcam
• Catalog: ab59543
• Prices: $403.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: FC WB ELISA IHC-F IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant full length protein (Rat) Peroxiredoxin 6
• Description: Rabbit Polyclonal
• Gene: PRDX6
• Conjugate: Unconjugated

Product images

ab59543 staining Peroxiredoxin 6 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.See Abreview

ab59543 at 1/100 dilution staining Peroxiredoxin 6 in astrocytes in Parkinson's disease by Immunohistochemistry. Secondary antibody anti-rabbit IgG conjugated to Cy3 (1/100).

ab59543 at 1/1000 dilution staining Peroxiredoxin 6 in astrocytes in Parkinson's disease by IHC-P. Secondary antibody Donkey anti-rabbit conjugated to biotin.

All lanes : Anti-Peroxiredoxin 6 antibody (ab59543) at 1/2500 dilutionLane 1 : Wild-type rat cortexLane 2 : Transgenic (APP/PS1) male mouse cortical lysateLane 3 : Transgenic (APP/PS1) male mouse cortical lysateLane 4 : Transgenic (APP/PS1) male mouse cortical lysateLane 5 : Transgenic (APP/PS1) male mouse cortical lysateLane 6 : Wild-type male mouse cortical lysateLane 7 : Wild-type male mouse cortical lysateLane 8 : Wild-type male mouse cortical lysateLane 9 : Wild-type male mouse cortical lysateSecondaryAlexa Fluor 680-conjugated goat anti rabbit IgG polyclonal antibody

ICC/IF image of ab59543 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59543, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab59543 staining Peroxiredoxin 6 in Human platelet cells by Flow cytometry.Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.See Abreview

Product References

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Synaptic protein expression is regulated by a pro-oxidant diet in APPxPS1 mice. - Synaptic protein expression is regulated by a pro-oxidant diet in APPxPS1 mice.

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Protection of peroxiredoxin II on oxidative stress-induced cardiomyocyte death - Protection of peroxiredoxin II on oxidative stress-induced cardiomyocyte death

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