Anti-Peroxiredoxin 6 antibody [4A3]
| Name | Anti-Peroxiredoxin 6 antibody [4A3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab16947 |
| Prices | $390.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 4A3 |
| Applications | FC ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Mouse, Rat, Human, Bovine, Ferret, Orangutan |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | PRDX6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Peroxiredoxin 6 antibody [4A3] (ab16947) at 1/1000 dilutionLane 1 : Cell lysates prepared from Hela cellsLane 2 : Cell lysates prepared from 293T cellsLane 3 : Cell lysates prepared from Jurkat cells](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10135_ab16947_1.JPG)
All lanes : Anti-Peroxiredoxin 6 antibody [4A3] (ab16947) at 1/1000 dilutionLane 1 : Cell lysates prepared from Hela cellsLane 2 : Cell lysates prepared from 293T cellsLane 3 : Cell lysates prepared from Jurkat cells
ICC/IF image of ab16947 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16947, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab16947 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16947, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10137_Peroxiredoxin-6-Primary-antibodies-ab16947-2.jpg)
Overlay histogram showing HeLa cells stained with ab16947 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16947, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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