All lanes : Anti-PFDN5 antibody [EPR7755] (ab129116) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : Fetal brain lysateLane 4 : Fetal heart lysateLane 5 : Fetal kidney lysateLane 6 : Human spleen lysateLane 7 : HepG2 cell lysateLane 8 : NIH/3T3 lysateLane 9 : F9 cell lysateLane 10 : Fetal muscle lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution
ab129116, at a 1/100 dilution, staining PFDN5 in paraffin-embedded Human colon carcinoma tissue by immunohistochemistry.