All lanes : Anti-PFKL antibody [EPR11904] (ab181064) at 1/5000 dilutionLane 1 : HepG2 cell lysateLane 2 : A431 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-PFKL antibody [EPR11904] (ab181064) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution
Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) staining PFKL with ab181064 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Flow cytometric analysis of 2% paraformaldehyde-fixed C6 cells labeling PFKL with ab181064 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.