All lanes : Anti-PGAM2 antibody [EPR15193(B)] (ab183027) at 1/20000 dilutionLane 1 : Human skeletal muscle lysateLane 2 : Human fetal heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemistry analysis of paraffin embedded Human skeletal muscle tissue, labeling PGAM2 with ab183027 at 1/500 dilution. Counterstained with Hematoxylin.
Immunopreciptation analysis of Human skletal muscle lysate, labeling PGAM2 with ab183027 at 1/50 dilution (lane 1) and negative control (lane 2) Anti-Rabbit IgG (HRP) secondary antibody used at 1/1500 dilution (lane 1) and blocking buffer was 5% NFDM/TBST.
Flow cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde, labeling PGAM2 with ab183027 at 1/20 dilution. Goatanti Rabbit IgG secondary antibody used at 1/150 dilution.