All lanes : Anti-PGAM2 antibody [EPR15194(B)] (ab187147) at 1/10000 dilutionLane 1 : Human skeletal muscle tissue lysateLane 2 : Human fetal heart tissue lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-PGAM2 antibody [EPR15194(B)] (ab187147) at 1/10000 dilutionLane 1 : Rat skeletal muscle tissue lysateLane 2 : Mouse skeletal muscle tissue lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue, labeling PGAM2 with ab187147 at 1/4000 dilution. Counterstained with Hematoxylin.
Western blot analysis of PGAM2 in immunoprecipitation pellets from Human skeletal muscle lysate. ab187147 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.