All lanes : Anti-PGBD1 antibody [EPR13883] (ab180598) at 1/10000 dilutionLane 1 : HeLa lysateLane 2 : Jurkat lysateLane 3 : 293 lysateLane 4 : K562 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution
Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling PGBD1 with ab180598 at a 1/500 dilution (left image), and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 488) secondary used at a 1/200 diution
Flow cytometry analysis of HeLa cells using ab180598 at a 1/20 dilution (red) or a rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.