All lanes : Anti-PGK1 antibody (ab154613) at 1/1000 dilutionLane 1 : A549 whole cell lysateLane 2 : HeLa whole cell lysateLysates/proteins at 30 µg per lane.
Anti-PGK1 antibody (ab154613) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
All lanes : Anti-PGK1 antibody (ab154613) at 1/1000 dilutionLane 1 : HeLa whole cell lysate (untreated)Lane 2 : HeLa whole cell lysate (1% O2 treatment for 24hr)Lysates/proteins at 30 µg per lane.
Anti-PGK1 antibody (ab154613) at 1/1000 dilution + Rat liver whole cell lysate at 50 µg
Immunohistochemical analysis of paraffin-embedded Hepatoma, labeling PGK1 using ab154613 at 1/500 dilution.
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PGK1 using ab154613 at 1/50 dilution. Right panel costained with Hoechst 33342.