Anti-PGLS antibody (ab83867) at 1 µg/ml (5% skim milk / PBS buffer) + 721_B cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab83867 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83867, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab83867 at 5 µg/ml staining PGLS in Human tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections.
ab83867 at 5 µg/ml staining PGLS in Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).