Anti-PGP9.5 antibody [13C4 / I3C4]

• Supplier: Abcam
• Catalog: ab8189
• Prices: $400.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: 13C4 / I3C4
• Applications: ICC/IF ICC/IF ICC/IF IHC-F IHC-F IHC-P WB
• Species Reactivities: Mouse, Rat, Sheep, Rabbit, Guinea Pig, Dog, Human, Pig, Zebrafish
• Antigen: PGP 9
• Description: Mouse Monoclonal
• Gene: UCHL1
• Conjugate: Unconjugated

Product images

ab8189 (1/20) immunostaining neurons in mouse cortical primary cell culture.

IHC-P image of PGP9.5 staining on zebrafish brain using ab8189 (1:1000). The sections were subjected to heat mediated antigen retrieval using citric acid. The sections were then blocked using 1% BSA for 10 mins for 21°C. The primary antibody (ab8189) was incubated at a dilution of 1:1000 at 21°C for 16 hours. The secondary antibody used was undiluted goat polyclonal to Mouse IgG conjugated to biotin.See Abreview

IHC-P image of PGP9.5 staining on P5 mouse skin sections using ab8189 (1:1000). The sections were de paraffinized and subjected to heat meadiated antigen retrieval using citric acid. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab8189 was then incubated for 16 hours at 21°C. the secondary antibidy used was Got polyclonal to anti mouse IgG conjugated to biotin (1:200). See Abreview

IHC image of PGP9.5 staining in Rat pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8189, 0.02µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) at 5 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Rat) Tissue Lysate Lane 3 : Brain (Mouse) Tissue LysateLane 4 : Rat Cortex Tissue Lysate Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 6 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

Alterations in the ubiquitin proteasome system in persistent but not reversible - Alterations in the ubiquitin proteasome system in persistent but not reversible

Beeken M, Lindenmeyer MT, Blattner SM, Radon V, Oh J, Meyer TN, Hildebrand D, Schluter H, Reinicke AT, Knop JH, Vivekanandan-Giri A, Munster S, Sachs M, Wiech T, Pennathur S, Cohen CD, Kretzler M, Stahl RA, Meyer-Schwesinger C. J Am Soc Nephrol. 2014 Nov;25(11):2511-25.

Changes in cardiac innervation during maturation in long-term diabetes. - Changes in cardiac innervation during maturation in long-term diabetes.

Bakovic M, Juric Paic M, Zdrilic E, Vukojevic K, Ferhatovic L, Marin A, Filipovic N, Grkovic I, Puljak L. Exp Gerontol. 2013 Dec;48(12):1473-8.

Alterations of calcium channels and cell excitability in intracardiac ganglion - Alterations of calcium channels and cell excitability in intracardiac ganglion

Liu J, Tu H, Zheng H, Zhang L, Tran TP, Muelleman RL, Li YL. Am J Physiol Cell Physiol. 2012 Apr 15;302(8):C1119-27. doi:

Homeodomain interacting protein kinase 2 regulates postnatal development of - Homeodomain interacting protein kinase 2 regulates postnatal development of

Chalazonitis A, Tang AA, Shang Y, Pham TD, Hsieh I, Setlik W, Gershon MD, Huang EJ. J Neurosci. 2011 Sep 28;31(39):13746-57.

Gastrointestinal tract disorder in natriuretic peptide receptor B gene mutant - Gastrointestinal tract disorder in natriuretic peptide receptor B gene mutant

Sogawa C, Abe A, Tsuji T, Koizumi M, Saga T, Kunieda T. Am J Pathol. 2010 Aug;177(2):822-8.

A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional - A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional

Theodorou E, Dalembert G, Heffelfinger C, White E, Weissman S, Corcoran L, Snyder M. Genes Dev. 2009 Mar 1;23(5):575-88.

Gastric neuroendocrine carcinomas in bearded dragons (Pogona vitticeps). - Gastric neuroendocrine carcinomas in bearded dragons (Pogona vitticeps).

Ritter JM, Garner MM, Chilton JA, Jacobson ER, Kiupel M. Vet Pathol. 2009 Nov;46(6):1109-16.

Interstitial cells of Cajal in the urethra are cGMP-mediated targets of nitrergic - Interstitial cells of Cajal in the urethra are cGMP-mediated targets of nitrergic

Garcia-Pascual A, Sancho M, Costa G, Triguero D. Am J Physiol Renal Physiol. 2008 Oct;295(4):F971-83. doi:

Immunohistochemical localization of galanin receptors (GAL-R1, GAL-R2, and - Immunohistochemical localization of galanin receptors (GAL-R1, GAL-R2, and

Arciszewski MB, Barabasz S, Calka J. Ann Anat. 2008;190(4):360-7.

Expression and function of rat urothelial P2Y receptors. - Expression and function of rat urothelial P2Y receptors.

Chopra B, Gever J, Barrick SR, Hanna-Mitchell AT, Beckel JM, Ford AP, Birder LA. Am J Physiol Renal Physiol. 2008 Apr;294(4):F821-9. doi: