![Overlay histogram showing SH-SH5Y cells stained with ab80664 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80664, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10656_ab80664-204500-ab80664FC.jpg)
Overlay histogram showing SH-SH5Y cells stained with ab80664 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80664, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-PGP9.5 antibody [35] (ab80664) at 1/1000 dilution + HEK293](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10657_ab80664-199771-ab80664.jpg)
Anti-PGP9.5 antibody [35] (ab80664) at 1/1000 dilution + HEK293
![Anti-PGP9.5 antibody [35] (ab80664) at 1/1000 dilution + recombinant UCHL1/3](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10658_UCHL13-Primary-antibodies-ab80664-2.jpg)
Anti-PGP9.5 antibody [35] (ab80664) at 1/1000 dilution + recombinant UCHL1/3