All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Y79 lysateLane 3 : U-87 MG lysateLane 4 : SH SY5Y lysateLysates/proteins at 10 µg per lane.
ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilutionLane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain)Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1)Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control) Lysates/proteins at 100 µg per lane.SecondaryHRP conjugated goat anti-rabiit polyclonal at 1/10000 dilutiondeveloped using the ECL technique
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD