Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PHACS with ab180949 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Anti-PHACS antibody [EPR8337] (ab180949) at 1/2000 dilution + HeLa cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-PHACS antibody [EPR8337] (ab180949) at 1/1000 dilution + Fetal heart lysate at 20 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution