Anti-PHACTR4 antibody [EP12543] (ab192882) at 1/10000 dilution + SH-SY5Y cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-PHACTR4 antibody [EP12543] (ab192882) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : Y79 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PHACTR4 with ab192882 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.
Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling PHACTR4 using ab192882 at a 1/180 dilution (red). Goat anti rabbit IgG (FITC) was used as the secondary antibody at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control cells without incubation with primary and secondary antibody (blue).