All lanes : Anti-PHF20 antibody (ab37605) at 1/250 dilutionLane 1 : Brain (Human) Tissue LysateLane 2 : Testis (Human) Tissue Lysate Lane 3 : Liver (Human) Tissue LysateLane 4 : Heart (Human) Tissue LysateLane 5 : Skeletal Muscle Normal (Human) Tissue Lysate Lane 6 : Blood Vessel: artery (Human) Tissue Lysate (ab28992)Lane 7 : Colon (Human) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.