Anti-PHGDH antibody
| Name | Anti-PHGDH antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57030 |
| Prices | $388.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF IHC-P FC IP |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-534 of Human PHGDH |
| Description | Mouse Monoclonal |
| Gene | PHGDH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
PHGDH antibody (ab57030) used in immunofluorescence at 1-5ug/ml on HeLa cells.
PHGDH antibody (ab57030) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymph node.
PHGDH was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10µg of Mouse monoclonal to PHGDH and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab57030.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.Band: 57kDa; PHGDH.
![Overlay histogram showing HeLa cells stained with ab57030 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57030, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/11042_PHGDH-Primary-antibodies-ab57030-2.jpg)
Overlay histogram showing HeLa cells stained with ab57030 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57030, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Differential expression of enzymes associated with serine/glycine metabolism in - Differential expression of enzymes associated with serine/glycine metabolism in
Kim SK, Jung WH, Koo JS. PLoS One. 2014 Jun 30;9(6):e101004.
Oncogene activation induces metabolic transformation resulting in - Oncogene activation induces metabolic transformation resulting in
Bollig-Fischer A, Dewey TG, Ethier SP. PLoS One. 2011 Mar 17;6(3):e17959.