Anti-PHGDH antibody - N-terminal (ab135772) at 1/50 dilution + Mouse brain tissue lysate at 35 µg
All lanes : Anti-PHGDH antibody - N-terminal (ab135772) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the PHGDH geneLysates/proteins at 2 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labelling PHGDH with ab135772 at 1/50 dilution, followed by peroxidase conjugated secondary antibody and DAB staining.
Flow cytometric analysis of HeLa cells labelling PHGDH with ab135772 at 1/10 dilution (bottom histogram), compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.