Anti-PI 3 Kinase catalytic subunit alpha antibody
| Name | Anti-PI 3 Kinase catalytic subunit alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5453 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ELISA WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to amino acids 1034-1050 of Human PI3KC A |
| Description | Rabbit Polyclonal |
| Gene | PIK3CA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab5453 staining PI 3 Kinase catalytic subunit alpha in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ICC/IF image of ab5453 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5453 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.