Anti-PI 3 Kinase catalytic subunit alpha antibody [4F3] (ab126819) at 1/500 dilution + Recombinant Human PIK3CA protein (AA: 881-1068)
All lanes : Anti-PI 3 Kinase catalytic subunit alpha antibody [4F3] (ab126819) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 cell lysate
Example of standard curves obtained using ab126819. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red).
Immunofluorescence analysis of HeLa cells using ab126819 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of Jurkat cells using ab126819 at 1/200 (green) and negative control (red).