Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388]

• Supplier: Abcam
• Catalog: ab32089
• Prices: $99.00, $379.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: Y388
• Applications: WB FC IP
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues in the N terminal of Human PI 3 Kinase catalytic subunit gamma
• Description: Rabbit Monoclonal
• Gene: PIK3CG
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab32089 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32089, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089) at 1/1000 dilution + Jurkat cell lysate.

Product References

PI3K p110gamma overexpression in idiopathic pulmonary fibrosis lung tissue and - PI3K p110gamma overexpression in idiopathic pulmonary fibrosis lung tissue and

Conte E, Gili E, Fruciano M, Korfei M, Fagone E, Iemmolo M, Lo Furno D, Giuffrida R, Crimi N, Guenther A, Vancheri C. Lab Invest. 2013 May;93(5):566-76.

Inhibition of PI3K prevents the proliferation and differentiation of human lung - Inhibition of PI3K prevents the proliferation and differentiation of human lung

Conte E, Fruciano M, Fagone E, Gili E, Caraci F, Iemmolo M, Crimi N, Vancheri C. PLoS One. 2011;6(10):e24663.