Anti-PI 3 Kinase p85 alpha antibody (ab63040) at 1/63 dilution + CEM cell line lysates at 35 µg
Anti-PI 3 Kinase p85 alpha antibody (ab63040) at 1/63 dilution + Hela cell line lysates at 35 µg
All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab63040) at 1/60 dilutionLane 1 : 293 cell line lysates nontransfectedLane 2 : 293 cell line lysates transiently transfected with the PI 3 Kinase p85 alpha geneLysates/proteins at 3 µg per lane.
ab63040, at 1/10-1/50 dilution, staining PI 3 Kinase p85 alpha in formalin-fixed, paraffin-embedded human skeletal muscle tissue by immunohistochemistry. ab63040 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.