All lanes : Anti-PIG3 antibody (ab64798) at 1 µg/mlLane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab3950)Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 3 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 5 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate Lane 6 : Lung (Human) Tissue Lysate Lane 7 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 8 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.