Anti-Pin1 antibody [EP1479Y]
| Name | Anti-Pin1 antibody [EP1479Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab76309 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1479Y |
| Applications | WB ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues near the N terminal of human Pin1 |
| Description | Rabbit Monoclonal |
| Gene | PIN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/500 dilution + 293 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/12058_ab76309.jpg)
Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/500 dilution + 293 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Pin1 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to Pin1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76309.Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.Band: 18kDa: Pin1.
Overlay histogram showing HeLa cells stained with ab76309 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76309, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
The cellular peptidyl-prolyl cis/trans isomerase Pin1 regulates reactivation of - The cellular peptidyl-prolyl cis/trans isomerase Pin1 regulates reactivation of
Guito J, Gavina A, Palmeri D, Lukac DM. J Virol. 2014 Jan;88(1):547-58.
Prolyl isomerase Pin1 promotes amyloid precursor protein (APP) turnover by - Prolyl isomerase Pin1 promotes amyloid precursor protein (APP) turnover by
Ma SL, Pastorino L, Zhou XZ, Lu KP. J Biol Chem. 2012 Mar 2;287(10):6969-73.
The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate - The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate
Krishnan N, Lam TT, Fritz A, Rempinski D, O'Loughlin K, Minderman H, Berezney R, Marzluff WF, Thapar R. Mol Cell Biol. 2012 Nov;32(21):4306-22.